Detection and analysis method of ferulic acid(1)

1. High performance liquid chromatography

The content of Ferulic Acid was determined by HPLC. The method is simple, rapid, accurate and precise. It is introduced in the literature that the mobile phase is mostly acid system, mainly including methanol water phosphoric acid system, methanol water glacial acetic acid system, methanol acetonitrile water glacial acetic acid system, etc. the amount of methanol can be adjusted properly in the experiment. The content of ferulic acid in Compound Ginkgo Oral Liquid was determined by HPLC. The Mixed Tocopherols OilFerulic Acid was methanol: 1% glacial acetic acid (45:55). The detection wavelength was 320nm, the flow rate was 1.0ml/min, and the column temperature was 25 ℃. The linear relationship of ferulic acid oxygen intake was good in the range of 0.176-0.88ug.

2. Thin layer scanning

Thin N-Acetylneuraminic Acid is also one of the commonly used methods for the determination of ferulic acid. The method is rapid, but its sensitivity is not ideal. Ji Shengguo et al. Determined the content of ferulic acid in Jiangzhi Tongmai oral liquid by TLC scanning. Using Flax lignans acetic acid chloroform (6:0.5:3.5) as developing agent, the single wavelength reflection hair saw tooth scanning was carried out with the scanning wavelength of 325nm. Good stability.